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. 2019 Mar 21;30(7):887–898. doi: 10.1091/mbc.E18-08-0545

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© 2019 Holenstein et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 2: — Analysis of immunofluorescence images. (A) Confocal images of the different cell lines stained with NucBlue (blue channel in the top left panel), phalloidin (red channel), and anti-vinculin (green channel) were used to obtain cell spreading area (top right panel), projected area of the nucleus (bottom left panel), and FA number (bottom right panel). (B) For volume estimations, nonadherent cells were stained with phalloidin (green channel) and NucBlue (red channel). In the example, confocal slices of a free-floating SaOs-2 cell (top panel) were reconstructed and segmented to estimate cytoplasmic and nuclear volumes (bottom panel). Scale bars: 25 μm.