Table 3.
M227‐specific variations resulting from comparative genomic analyses of the three strains belonging to Psa3 clade 2.
| No. | Variant type | Nucleotide /sequence in M227 | Affected genesa | Products | Primers for Sanger sequencing |
|---|---|---|---|---|---|
| 1 | SNP | g → a | IYO_RS06590, P29S | Hypothetical protein | PSA184/185 |
| 2 | SNP | g → a | IYO_RS21050, M87I | DNA ligase‐associated DEXH box helicase | PSA188/189 |
| 3 | SNP | a → g | IYO_RS24010, RBS | DNA‐binding response regulator | PSA194/195 |
| 4 | Deletion | ‘cgggcgaac’ absence | IYO_RS25325, 3AA deletion | RNA‐binding protein Hfq | hfq‐orf‐F/R |
| 5 | Deletion | ‘gcgtcgccgt’ absence | IYO_RS02715, frame shift | DNA‐directed RNA polymerase subunit beta | PSA176/177 |
| 6 | Insertion | 1176‐bp DDE locus | At locus 930 bp upstream of hrpR | ‐ | PSA198/199 |
| 7 | Insertion | gg | Non‐coding sequence within the integrative and conjugative element (ICE) | ‐ | PSA200/201 |
| 8 | Insertion | 106 bp | IYO_RS24140, truncated | Lytic transglycosylase | PSA202/203 |
a
Genetic loci are designated according to the genome information of ICMP 18884 (NZ_CP011972).