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. 2019 Apr 1;30(8):1008–1019. doi: 10.1091/mbc.E18-09-0579

FIGURE 2:

FIGURE 2:

Arl1 and Imh1 act interdependently to suppress defects in endosome-to-TGN transport and temperature-sensitive growth in ypt6Δ cells. (A) Arl1 and Imh1 require each other to suppress the transport defects in ypt6Δ cells. Arl1 or Imh1 was coexpressed with GFP-Snc1 in the indicated cells. Live cells were observed in mid–log phase using fluorescence microscopy. Cells showing the GFP-Snc1 plasma membrane signal were quantified (n = 100). The data are reported as the mean ± SD of three independent experiments (***p < 0.001; N.S. not significant; one-way ANOVA; scale bar, 5 μm). (B) Both Arl1 and Imh1 are required to suppress temperature-sensitive growth defects in ypt6arl1Δ and ypt6imh1Δ cells. Yeast strains transformed with Arl1 or Imh1 were serially diluted 10-fold as indicated, spotted on plates, and incubated at 30 or 37°C. (C) Cytosolic distribution of Arl1 and Imh1 in ypt6Δ cells can be restored by overexpressing Imh1 or Arl1, respectively, in an Arl3-dependent manner. Arl1-mRFP or mCherry-Imh1 was coexpressed with Imh1 or Arl1, respectively, in the indicated cells. Live cells were observed in mid–log phase using fluorescence microscopy (scale bar, 5 μm).