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. 2019 Apr 1;30(8):1008–1019. doi: 10.1091/mbc.E18-09-0579

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© 2019 Chen, Wang, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 5: — The N-terminal region of Imh1 is critical for recruiting the GARP complex to the TGN in the absence of Ypt6. (A) Schematic diagram of Imh1^dN100 and mCherry-Imh1 constructs. (B) The N-terminal region of Imh1 is required for restoring GARP complex localization in ypt6Δ cells. GFP-tagged Vps52 or Vps53 was coexpressed with wild-type Imh1, Imh1^dN100, or mCherry-Imh1. Cells containing Vps52-GFP and Vps53-GFP puncta were quantified (n = 150). The data are reported as the mean ± SD of three biological repeats (***p < 0.001; N.S. not significant; one-way ANOVA; scale bar, 5 μm). (C) The N-terminal region of Imh1 is not involved in the localization of Arl1-GFP. Different forms of Imh1 were coexpressed with Arl1-GFP in ypt6arl1Δ cells to examine the localization of Arl1. Live cells were observed in mid–log phase using fluorescence microscopy (scale bar, 5 μm).