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. 2019 Apr 1;30(8):975–991. doi: 10.1091/mbc.E18-12-0774

FIGURE 1:

FIGURE 1:

Fruit extracts induce invasive filamentation in S. japonicus. (A) S. japonicus growing in agar plates on solid rich media (YE), supplemented or not with 10% fruit extracts before (top) and after (middle) washing of the plate, as well as the same plates imaged under a stereomicroscope (bottom). (B) S. japonicus growing on solid rich media supplemented or not with a range of concentration of RGE and the same plates under the stereomicroscope. (C) S. japonicus, S. pombe, S. octosporus, and S. cerevisiae growing on solid rich media (YE or YPD) and supplemented or not with RGE before (top) and after (bottom) washing of the plate. (D) Tropism experiment to assess the directionality of S. japonicus hyphal growth. White filter squares were soaked with YE (top) or RGE (bottom). (E) Ratio of positive vs. negative growth tropism in experiments such as in D. Positive tropism denotes growth toward and negative tropism away from the filter; p = 0.09, t test. Error bars show standard deviations. Dotted lines highlight penetrative filamentous growth.