FIGURE 8:

DBM shows an effect on small eIF2B bodies similar to that of ISRIB. (A) FRAP analysis was carried out on eIF2α-GFP localized to small eIF2B bodies in U373 cells transfected with eIF2α-GFP and eIF2Bε-RFP to mark the eIF2B bodies. Cells were treated with 20 µM DBM. FRAP analysis was performed on 10 bodies (n = 3), and the percentage of eIF2 that recovered is presented as mean ± SEM. (B) U373 cells were fixed in methanol and subject to ICC with a primary anti-eIF2Bδ antibody and visualized using an appropriate secondary antibody conjugated to Alexa Fluor 568. Within a population of 50 cells, the median percentage of colocalization between anti-eIF2Bδ and large and medium (L and M) or small (S) eIF2Bε-GFP bodies (n = 3) is shown. p Values derived from a Kruskal-Wallis test, followed by a Conover-Inman analysis, *p ≤ 0.05.