Figure 1.

Offspring timeline and maternal obesity cohort design. (A) Male offspring of mothers were weaned on HFD or chow diet at 4 weeks of age until cull (33 weeks). At 31 weeks of age, a subgroup of offspring from both maternal diet groups was injected (i.p.) with NMN daily. (B) Mothers were fed with either chow diet (C) or HFD (H). Offspring of both maternal groups were fed with either chow diet (CC and HC) or HFD (CH and HH). Nicotinamide mononucleotide (NMN) was injected, intraperitoneally, in four groups (CCN, CHN, HCN and HHN). Phosphate buffered saline vehicle was injected in the other four groups (CCV, CHV, HCV, HHV).