Table 1.

The Sequence of mutant alleles in SNX5-knockout single cell clones. PAM sequences are labeled by underlines.

WT: 5'-GAGAGACAAAGTCAAATTTACAGTGCACA• CAAAGGTAAGTAACATGCAGTGGTAATACAT-3'
SNX5-KO_1: GAGAGACAAAGTCAAATTTACAGTGCACAACAAAGGTAAGTAACATGCAGTGGTAATACAT +1 bp
GAGAGACAAAGTCAAATTTACAGTGCACAACAAAGGTAAGTAACATGCAGTGGTAATACAT +1 bp
WT: 5'-CCTTGTTTCTGCAGATGTTGCCGATG• ACTATATCCACACCGCAGCCTGCTTACATAGCC-3'
SNX5-KO_2.1: CCTTGTTTCTGCAGATGTTGCCGATG• • • TATATCCACACCGCAGCCTGCTTACATAGCC -2 bp
CCTTGTTTCTGCAGATGTTGCCGATGAACTATATCCACACCGCAGCCTGCTTACATAGCC +1 bp
SNX5-KO_2.2: CCTTGTTTCTGCAGATGTTGCCGATGAACTATATCCACACCGCAGCCTGCTTACATAGCC +1 bp
CCTTGTTTCTGCAG • • • • • • • • • • • • • • • • • • • • • • • ACCGCAGCCTGCTTACATAGCC -23 bp
WT: 5'-AGTTAACTTCTTTCTTTCTTTACAGACCACAC• TGCCCACGTTTCAGAGCCCAGAGTTTT-3'
SNX5-KO_3: AGTTAACTTCTTTCTTTCTTTACAGACCACACTTGCCCACGTTTCAGAGCCCAGAGTTTT +1 bp
AGTTAACTTCTTTCTTTCTTTACAGACCACACTTGCCCACGTTTCAGAGCCCAGAGTTTT +1 bp