Figure 1.

OP-B inhibits the migration of A549 cells co-cultured with LPS-challenged THP-1 cells. (A~B), Levels of Stat3 and Stat5 were detected by western blot assay with specific antibodies, β-actin was used as a control. (C~D), Effects of OP-B on the migration of cells across the Transwell chamber in the co-culture system. Images are representative of three independent experiments. (E~F), Effects of OP-B on the levels of EMT-related markers in co-cultured A549 cells. Levels of E-cadherin, ZO-1, N-cadherin, ZEB1, and Vimentin were detected by western blot assay, β-actin was used as a loading control. Error bars represented mean ± SD from three independent experiments. (*), P < 0.05; (**), P < 0.01; (***), P < 0.001.