Fig. 2.

Western blot of dimer formation by diamide oxidation of recombinant γD-crystallin and cysteine mutants in Chelex treated 50 mM potassium phosphate (pH 7.4). Protein was incubated with 10 μM of diamide at 37 °C for 3h and disulfide bond formation was analyzed by western blot using hγD polyclonal antibody and the blot was developed using X-ray film using ECL solution the developed film was scanned under greyscale using Epson scanner. For both reduced and non-reduced blot samples were derived from the same experiment and blots were processed in parallel.