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. Author manuscript; available in PMC: 2019 Jun 24.
Published in final edited form as: Eur J Neurosci. 2018 Nov 29;49(1):27–39. doi: 10.1111/ejn.14223

FIGURE 6.

FIGURE 6

Inhibition of CaMKK β activity increased leukocyte adhesion to endothelial cells under OGD with reoxygenation conditions. The leukocyte-endothelium adhesion assay was performed to analyze the anti-inflammatory effects mediated by CaMKK β activity. (a) Representative optical microscopy images of HBEC-5i endothelial cell monolayers and binding leukocytes (THP-1 cells) treated with DMSO (0.1%) or STO-609 (10 μM) under normoxic conditions (Norm) or 18-hr OGD with 24-hr reoxygenation (OGD + Reox). (b) Fluorescence intensity of the bound calcein-AM labeled leukocytes was quantitated after the same treatments as (a). DMSO-treated cells under normoxic conditions were considered control (Ctrl). Data are expressed as the means ± SEM of five independent experiments. **p < 0.01, ***p < 0.001 versus control cells. ###p < 0.001 versus DMSO-treated cells under OGD + Reox conditions. Not significant (ns) between indicated treatments. Statistical analyses were performedusing one-way ANOVA and the Holm-Sidak multiple comparison test. [Colour figure can be viewed at wileyonlinelibrary.com]