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. Author manuscript; available in PMC: 2019 Jun 24.
Published in final edited form as: Eur J Neurosci. 2018 Nov 29;49(1):27–39. doi: 10.1111/ejn.14223

FIGURE 7.

FIGURE 7

CaMKK β inhibition by STO-609 increases cerebrovascular permeability and endothelial cell apoptosis in vivo. (a) Evans blue extravasation was carried out to evaluate the cerebrovascular permeability. (b) STO-609 treatment exacerbated endothelial cell apoptosis in the penumbra 24 hr after MCAO. CD31 positive signal was shown in green color and cleaved caspase-3 was shown in red color. Scale bar: 20 μM. Mice were treated with DMSO or STO-609 1-hr before 90 min of middle cerebral artery occlusion (MCAO) and brains were harvested 24 hr after MCAO. BBB permeability data are expressed as mean ± SEM (n = 4 for sham group, n = 5 for MCAO + DMSO group and n = 6 for MCAO + STO-609 group). *p < 0.05, ***p < 0.001 versus sham group. ##p < 0.05 versus MCAO + DMSO group. Statistical analyses were performed by one-way ANOVA tests followed by Holm-Sidak multiple comparison test. [Colour figure can be viewed at wileyonlinelibrary.com]