C57BL/6J mice were retro-orbitally inoculated with 100,000 syngeneic TIB-49 AML cells that were stably transduced with GFP. The mice were then treated with 1mg/kg SGI-110 or diluent control. At day 12 following inoculation, BM and spleen cells were isolated and evaluated using flow cytometric analysis for (A) BM engraftment, (B) PD-1 expression of CD4+ cells in the spleen, (C) Myeloid-derived suppressor cell (MDSC) burden in the bone marrow and spleen, and (D) intracellular IFN-γ expression after exposure to autologous tumor lysate in the bone marrow and spleen.