Fig. 5.

miR-942 promotes HSC activation, fibrogenesis via BAMBI downregulation. a α-SMA, Col1A1, and MMP9 mRNA expression in hHSC, as measured by quantitative real-time PCR after transfection with miR-942 mimics, control mimics or BAMBI expression plasmid (pBAMBI) for 48 h. Data represent mean ± SD of three independent experiments, n = 3 per group. b α-SMA, Col1A1, and BAMBI expression in LX2 cells, as determined by immunoblot after transfection with miR-942 mimics, control mimics or BAMBI expression plasmid (pBAMBI) for 72 h. c Expression of α-SMA, Col1A1, and TIMP1 in hHSCs, as determined by real-time PCR after transfection with miR-942 inhibitor, control inhibitor, si-BAMBI or a si-control for 72 h. Data represent mean ± SD of three independent experiments, n = 3 per group. One-way ANOVA (and nonparametric) Kruskal–Wallis test was used for statistical comparison of data. d α-SMA, Col1A1, and BAMBI expression, as determined by immunoblot in LX2 cells, after transfection with miR-942 inhibitor, control inhibitor, si-BAMBI or si-control for 72 h