Figure 3.
Survival fraction of S. aureus incubated with TBO followed by irradiation. A value of 1 was assigned to the survival fraction of the control untreated cells. (A) Effect of different TBO concentrations on the survival of bacterial cells irradiated by the red light for 20 minutes (TBO concentration: 20, 40, 60, and 80 μM). (B) survival fraction as a function of irradiance (irradiance: 0.68, 1.07, 2.47, 5.27, and 7.30 mW/cm2; irradiation duration was 20 minutes). (C) effect of irradiation duration on phototoxicity of TBO against bacterial cells. Cells were irradiated with light for 5, 10, 20, 30, and 40 minutes.