Figure 5.

Treatment with EAE sera from MOG immunized rats leads to increased expression of αB‐crystallin in primary oligodendrocyte cultures. Immunocytochemistry showing mature oligodendrocytes expressing (a) CNPase and (b) MOG (red), co‐stained with Olig2 (green). Immunocytochemistry of oligodendrocytes with CNPase (red) and αB‐crystallin (cryαB; green) following (c) no treatment, or 90 min in (d) 100 μM H₂O₂ and (e) 100 μM Sin‐1 as positive controls for stress. Treatment with sera from (f, h) sham and (g, i) MOG immunized rats led to increased expression of cryαB (green). (j) Western blot and quantification revealed significant elevation of cryαB in MOG sera‐treated cultures than sham (n = 5 treated cultures for 1% serum treatment and n = 3 treated cultures for 10% serum treatment). Scale bar = 50 μm. *p < .05, Student's t‐test