Figure 3.

Overexpression of STOX1 regulated the PI3K/AKT signaling pathway in HTR‐8/SVneo cells. Cells were transfected with the STOX1 plasmid for 48 hours. A, The expression level of STOX1 was examined. B, The expressions of pAKT, p85, and AKT proteins were determined by Western blot analysis. C, The expressions of pAKT and AKT proteins were determined after the cells were treated with LY294003. D, Cells were treated with the STOX1 plasmid after pretreatment with LY294002. Cell proliferation was detected using MTT. **P < 0.01 versus shSTOX1‐independent treatment. STOX1, storkhead box 1