Table 5.
Detection of the H9 HA gene from artificially reconstituted clinical samples spiked with H9N2 virus
| Sample name | Type or subtype | Virus‐spike | Type A rRT‐PCR assay † | H9 rRT‐PCR assay † | Other type/subtype rRT‐PCR assays † , ‡ |
|---|---|---|---|---|---|
| F16‐9 | H1N1pdm09 | + | 21.05 | 31.56 | 20.59 |
| − | 21.06 | N.D. | 20.58 | ||
| F16‐26 | H1N1pdm09 | + | 20.62 | 31.62 | 20.74 |
| − | 20.64 | N.D. | 20.78 | ||
| F16‐61 | H1N1pdm09 | + | 23.88 | 31.21 | 23.25 |
| − | 23.01 | N.D. | 23.06 | ||
| F14‐53 | H3N2 | + | 19.13 | 31.68 | 18.54 |
| − | 19.06 | N.D. | 18.48 | ||
| F15‐7 | H3N2 | + | 18.30 | 31.84 | 17.84 |
| − | 19.31 | N.D. | 17.99 | ||
| F16‐17 | H3N2 | + | 16.73 | 31.72 | 16.31 |
| − | 17.45 | N.D. | 16.48 | ||
| F15‐15 | Type B | + | 32.61 | 32.42 | 23.12 |
| − | N.D. | N.D. | 22.77 | ||
| F16‐44 | Type B | + | 32.31 | 32.02 | 21.52 |
| − | N.D. | N.D. | 21.16 | ||
| F16‐56 | Type B | + | 33.32 | 31.35 | 18.75 |
| − | N.D. | N.D. | 18.90 | ||
| F16‐52 | − | + | 32.35 | 31.57 | N.T. |
| − | N.D. | N.D. | N.T. | ||
| F16‐68 | − | + | 32.64 | 31.70 | N.T. |
| − | N.D. | N.D. | N.T. | ||
| F16‐76 | − | + | 32.12 | 31.67 | N.T. |
| − | N.D. | N.D. | N.T. | ||
| PBS | − | + | 31.51 | 31.43 | N.T. |
| − | N.D. | N.D. | N.T. |
†
Crossing point values were determined using the second derivative maximum method in Light Cycler® 480 SW1.5 software.
‡
Other assays were as follows: H1pdm rRT‐PCR assay for F16‐9, F16‐26, and F16‐61; H3 rRT‐PCR assay for F14‐53, F15‐7, and F16‐17; B/NS rRT‐PCR assay for F15‐15, F16‐44, and F16‐56.
N.D., not detected; N.T., not tested; rRT‐PCR, real‐time RT‐PCR.