Figure 2.

Overview of the machine perfusion protocol. A, The machine perfusion protocol included 1 h of DHOPE, 1 h of COR, and subsequent NMP for at least 150 minutes. Each phase of machine perfusion served a different purpose as described in the upper part of the figure. Machine perfusion settings were adjusted according to the perfusion temperature. The temperature was kept at 10°C during DHOPE and was gradually increased to 37°C during the COR phase, after which the liver was functionally tested during NMP. PV and mean HA pressure were set at 5 and 25 mm Hg, respectively, during DHOPE and were gradually increased during COR to 10 and 70 mm Hg, respectively, at the start of NMP. DHOPE, dual hypothermic oxygenated machine perfusion; COR, controlled oxygenated rewarming; HA, hepatic artery; NMP, normothermic machine perfusion; PV, portal vein [Color figure can be viewed at wileyonlinelibrary.com]