Figure 3.

Reduced susceptibility of LAMP‐2‐deficient HeLa cells to metacyclic trypomastigote (MT) invasion. (a) HeLa cells depleted in either LAMP‐1 or LAMP‐2 as well as wild‐type (WT) control were incubated for 1 hr with MT. The amounts of intracellular parasites are shown as means ± standard deviation of three independent assays performed in duplicate. MT invasion was significantly diminished in cells deficient in LAMP‐2 (^* P < 0.005). (b) HeLa cells depleted in LAMP‐1 or LAMP‐2 were incubated for 30 min with MT and visualised by confocal microscopy, upon reaction with antibody to LAMP‐1 or LAMP‐2 as in Figure 2b, with 100× objective. Scale bar = 10 μm. Note that in the left panel, parasites internalised in cells expressing LAMP‐1 at higher levels (red arrows) and lower levels (orange arrows) or in LAMP1‐negative cells (white arrows). In the right panel, parasites internalised in a few LAMP‐2‐positive cells (red arrows) are shown