Figure 5.

PPI network suggests that PIP interacts with numerous components of the AKT/MAPK pathway. (a) PPI network for PIP was retrieved from the STRING database and reconstructed by adding recently identified PIP interacting proteins. (b) Western blot analyses in SCC15 and SCC25 cells after transfection with control, PIP vector, and siRNA‐PIP. β‐Actin served as an internal control. (c) Proposed model for the effects of PIP on OSCC cell proliferation, invasion, migration and AKT/MAPK signaling. AKT/MAPK: Akt/mitogen‐activated protein kinase; OSCC: oral squamous cell carcinoma; PIP: prolactin‐inducible protein; PPI: protein–protein interaction; siRNA: small interfering RNA [Color figure can be viewed at wileyonlinelibrary.com]