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. 2019 Jan 28;234(9):15330–15341. doi: 10.1002/jcp.28180

Figure 6.

Figure 6

Effects of siRNA‐PIP coculture and AKT/MAPK inhibitor on OSCC cell proliferation. (a) Cells treated with DMSO, MK‐2206, SCH772984, siRNA‐PIP + MK‐2206 or siRNA‐PIP + SCH772984 were assessed by colony forming assays as described in Figure 2(a). All data were shown as mean ± SD. *p < 0.05, **p < 0.01. (b) At 24, 48, and 72 hr posttreatment with DMSO, MK‐2206, SCH772984, siRNA‐PIP + MK‐2206 or siRNA‐PIP + SCH772984, cell proliferation was examined via MTT assays. (c) Cell cycle analysis of SCC15 and SCC25 cell cycle treated as in (a). All data were shown as mean ± SD. *p < 0.05, **p < 0.01. AKT/MAPK: Akt/mitogen‐activated protein kinase; DMSO: dimethyl sulfoxide; MTT: 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide; OSCC: oral squamous cell carcinoma; PIP: prolactin‐inducible protein; SD: standard deviation; siRNA: small interfering RNA [Color figure can be viewed at wileyonlinelibrary.com]