Figure 5.

Regulation of CXCL5 and its role in cell invasion and tumor formation. (A) CXCL5 secretion of cells was assessed by ELISA. (B) CXCL5 secretion in HLF/HLF‐T cells and those treated with 10 µM Ly for 72 hours, as well as in HLF‐Axl‐KO1 and HLF‐Axl‐KO1‐T cells. (C) Representative images of hepatospheres consisting of HLF/HLF‐T/HLF‐CXCL5 cells. (D) Quantitative analyses of respective hepatosphere invasion into collagen gels. (E) Volumes of HLF/HLF‐T/HLF‐CXCL5‐derived tumors. (F) Immunohistochemical analysis showing consecutive tumor sections of HLF‐T‐derived and HLF‐CXCL5‐derived tumors stained with anti‐CXCL5 or anti‐Flag antibody. The secondary antibody was used only as control. Error bars depict SD from three individual experiments carried out in triplicates. *P < 0.05, **P < 0.01, ***P < 0.001. Abbreviations: ELISA, enzyme‐linked immunosorbent assay; n.s., not significant.