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. 2019 Jun 7;10:596. doi: 10.3389/fphar.2019.00596

Figure 1.

Figure 1

Effects of six β-adrenoceptor ligands (10 µM each) and forskolin (100 µM) on cAMP accumulation. Stimulations were performed after 24 h in the absence (vehicle) or presence of PTX (100 ng/ml). Each data point represents a single experiment and data in the absence and presence of PTX within an experiment are connected by a line. Geometric means with confidence intervals of cAMP accumulation by the six ligands and forskolin in the absence of PTX were 262 (87; 793), 178 (62; 505), 221 (90; 543), 243 (89; 665), 66 (28; 153), 116 (55; 244), and 152 (68; 608) fmol/25 µl, respectively. Following pretreatment with PTX geometric means were 190 (56; 645), 222 (101; 490), 320 (182; 562), 223 (72; 696), 93 (38; 225), 133 (51; 347), and 126 (44; 358) fmol/25 µl, respectively.