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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: J Cell Biochem. 2019 Apr 8;120(8):14044–14054. doi: 10.1002/jcb.28679

FIGURE1.

FIGURE1

Compound 3289–8625 regulates Wnt signaling activity in HRMECs. HRMECs were treated with either LCM, WCM, or WCM in the presence of compound 3289–8625 (50 μM) for 24 hours. AXIN2 mRNA expression levels were measured by qPCR. HRMECs treated with compound 3289-8625 presented reduced Wnt signaling levels, indicating that Wnt signaling activity can be regulated by compound 3289–8625 in HRMECs. HRMEC, human retinal microvascular endothelial cell; LCM, L-cell control conditional medium; mRNA, messenger RNA; qPCR, quantitative polymerase chain reaction; WCM, Wnt-3a conditional medium