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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: J Cell Biochem. 2019 Apr 8;120(8):14044–14054. doi: 10.1002/jcb.28679

FIGURE 2.

FIGURE 2

Compound 3289–8625 suppressed β-catenin protein level, thus regulating Wnt activity in HRMECs. HRMECs were treated with LCM (A), WCM (B), or WCM in presence of compound 3289-8625 (50 μM) (C), for 24 hours. Five random fields were examined on the slide. The green channel of phalloidin staining represents the HRMECs cytoskeleton. Immunofluorescence analysis of total β-catenin protein levels, as indicated by the red channel in HRMECs, shows that WCM enhanced the translocation of β-catenin protein levels into the nucleus. Moreover, compound 3289-8625 can inhibit Wnt signaling activity in HRMECs. HRMEC, human retinal microvascular endothelial cell; LCM, L-cell control conditional medium; WCM, Wnt-3a conditional medium