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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: J Cell Biochem. 2019 Apr 8;120(8):14044–14054. doi: 10.1002/jcb.28679

FIGURE 3.

FIGURE 3

Wnt signaling inhibition reduces Wnt-3a- dependent migration in HRMECs. HRMECs were seeded into the μ-35 dishes with culture insert. Culture inserts were removed after 24 hours creating a uniformed cell-free surface. Cells were then treated with LCM, WCM, or WCM in the presence of compound 3289–8625 (50 μM). Cell images of five random views were taken after 16 hours (A). Image analysis was performed using ImageJ (B). These data show that Wnt-mediated HRMECs migration can be inhibited by compound 3289-8625 (**P 0.01, ****P 0.001 via one-way ANOVA with Dunnett’'s correction). ANOVA, analysis of variance; HRMEC, human retinal microvascular endothelial cell; LCM, L-cell control conditional medium; WCM, Wnt-3a conditional medium