Skip to main content
. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: J Cell Biochem. 2019 Apr 8;120(8):14044–14054. doi: 10.1002/jcb.28679

FIGURE 4.

FIGURE 4

Wnt signaling inhibition reduces Wnt-3a-dependent tube formation in HRMECs. HRMECs were seeded into Matrigel-coated six-well plate. These cells were then treated with LCM, WCM, orWCM in presence of compound 3289-8625 (50 μM). Cell images of five random views were taken after 6 hours (A) then analyzed by ImageJ (B). Wnt-induced HRMECs tube formation was inhibited by compound 3289–8625 (*P < 0.05 and ****P < 0.001 via one-way ANOVA with the Dunnett correction). ANOVA, analysis of variance; HRMEC, human retinal microvascular endothelial cell; LCM, L-cell control conditional medium; WCM, Wnt-3a conditional medium