Hydrogen peroxide (H2O2) increases β-catenin protein level activation in HRMECs and compound 3289–8625 attenuates these effects. HRMECs were treated with (A) vehicle, (B) H2O2 (1 μM), and (C) H2O2 (1 μM) presenting with compound 3289–8625 (50 μM), for 24 hours. Total β-catenin protein levels were detected by immunofluorescence, as shown by the red channel. The green channel of phalloidin staining represents the HRMECs cytoskeleton. Data suggests H2O2 upregulated Wnt activity in the HRMECs. Compound 3289–8625 can inhibit H2O2-induced Wnt activation. HRMEC, human retinal microvascular endothelial cell