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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: J Cell Biochem. 2019 Apr 8;120(8):14044–14054. doi: 10.1002/jcb.28679

FIGURE 8.

FIGURE 8

Inhibiting Wnt suppresses H2O2-induced HRMECs migration. HRMECs were seeded into the μ-35 dishes with culture-insert. Culture inserts were removed after 24 hours, creating a uniformed cell-free surface. Cells were then treated with vehicle, H2O2 (1 μM), or H2O2 (1 μM) presenting with compound 3289–8625 (50 μM). Images of five random views were taken after a 16-hour incubation (A) and then analyzed using ImageJ (B). Our analysis shows that H2O2-induced Wnt activation mediates HRMECs migration and this phenomenon is reduced with treatment of Wnt modulator, 3289-8625 (*P < 0.05 and **P < 0.01 via one-way ANOVA with the Dunnett correction). ANOVA, analysis of variance; HRMEC, human retinal microvascular endothelial cell