Figure 3.
(a) RNA levels of NOX1 and NOX2 quantified using RT-qPCR in patients without reflux (NR) and with reflux (R) and by the ages of the same groups. Results are expressed as arbitrary units. ∗p < 0.05, ∗∗p < 0.005. (b) Distribution of the percentage of patients with positive protein expression for NOX1 and NOX2 in patients without reflux (NR) and with reflux (R), and by age, n = number of patients. (c) Protein expression images of NOX1 in NR < 50 (A), NR ≥ 50 (B), R < 50 (C, D, E), and R ≥ 50 (F) patients. (d) Protein expression images of NOX2 in NR < 50 (A), NR ≥ 50 (B, C), R < 50 (D), and R ≥ 50 (E, F) patients. The arrows are the brown coloration indicating the specific precipitate that correlates with the expression of the said protein.