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. 2019 Jun 9;2019:7123078. doi: 10.1155/2019/7123078

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Copyright © 2019 Yujia Wang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Diversity analysis of DASC differentiation potency at single cell resolution. (a) qPCR analysis of stem cell markers KRT5, P63, CDH1, and SOX9 expressed in 6 different single-cell-derived DASC clones originated from 3 individuals. 2 clones (clone A and clone B) were generated from each individual. (b) Diagram showing the ratio of AQP5+, HOPX+, LAM3+, and CC10+ cells generated from different single-cell-derived clones after 12 days of monolayer differentiation. DASCs before monolayer differentiation (Undiffer) were used as a negative control. (c) AQP5+, HOPX+, LAM3+, and CC10+ cell frequencies in differentiated DASCs at different culture passages. S1: healthy subject; S2: subject with ILD; S3: subject with bronchiectasis.