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High LDL decreases NOS3 protein levels. Human primary endothelial cells were cultured in the standard medium (con) or medium containing 1 mg/ml LDL (high LDL) for 7 days. Full-length NOS3 protein was detected by immunoblot; ERK1/2 served as a loading control. (a) Representative immunoblots. (b) Semiquantitative analysis. Data are mean ± SEM; n = 7; p < 0.05 vs. con.
