Name	Company	Catalog Number	Comments
methylene blue	Kordon	B016CBHZUS	antifungal
heater	Finnex	4711457836017	100W Digital Control Heater
airstone	Lee's Aquarium & Pet Products	10838125202	disposable air stone
salt	Instant Ocean	51378014021	Sea Salt
nursery container	IPC	21545-002	40 oz or 1.5 L clear containers
transfer pipette	VWR	414004-002	plastic bulb pipettes
compact culture system(CCS) starter kit with Brachionus plicatilis (L-type) rotifers	Reed Mariculture	na	fish food
RGcomplete	APBreed	817656016572	32 oz bottle of rotifer food
Programmable Auto Dosing Pump DP-4	Jebao	DP-4	automatic feeder for rotifers
Tricane-S	Western Chemical	MS 222	fish anesthetic
sodium bicarbonate	Sigma-Aldrich	S5761-500G	for tricane solution
nylon mesh strainer	HIC (Harold Import Co.)	735343476235	3-inch diameter
Formalin solution, neutral buffered, 10%	Sigma-Aldrich	HT501128-4L	fixative
10X PBS	Invitrogen	AM9625	buffer, dilute to 1X using distilled water
Triton-x 100	Sigma-Aldrich	T8787-250ML	detergent
sodium azide	Sigma-Aldrich	S2002-25G	anti-bacterial
bovine serum albumin	Sigma-Aldrich	A9647-100G	blocking reagent
glass vial with screw-top cap 4mL	Wheaton	224742	staining vial
plastic mesh screen for breeding tank	Pentair	N1670	Cut into a rectangle 6mm larger on all edges than the dimensions of the bottom of the breeding tank. Cut a 6mm square from each corner of the rectangle. Bend the edges of the screen down along all four edges.Place a pair of 6mm vinyl-coated disk magnets on either side (top and bottom) of the mesh on each corner. The screen should be as snug as possible to the sides of the tank. The screen can be removed from the tank with a metal fish net.
vinyl-coated disk magnets	Kjmagnets	D84PC-AST
New Life Spectrum Thera-A pellet fish food	New Life International	na	Adult fish food. A list of retailers for this product is available on the company website
Antibodies
insulin antibody from guinea pig	Dako	A0564	1:200
glucagon antibody from sheep	Abcam	ab36215	1:200
acetylated tubulin antibody from mouse	Sigma	T6793	1:500
HuD/HuC antibody from mouse	Life Technologies	A-21271	1:500
nitric oxide synthase (nNOS) antibody from rabbit	Abcam	ab106417	5μg/mL
choline acetyltransferase (ChAT) from rabbit	Abcam	ab178850	1:2000
seratonin (5HT) from rabbit	Immunostar	20080	1:500