Table 7.

Brain Stability of Analytes Under Study

	Long term (10 days, −20°C)			Short term (12 h, 9°C)			Bench top (6 h, 25°C)			Freeze-thaw (3 cycles, −20°C)
										Cycle 1			Cycle 2			Cycle 3
pmol	5	50	500	5	50	500	5	50	500	5	50	500	5	50	500	5	50	500
THC
Mean±SD	494.08±8.44	48.49±1.21	494.08±8.44	5.47±0.12	52.98±0.90	569.20±7.79	4.58±0.21	45.88±0.49	577.09±4.94	4.86±0.14	46.77±0.29	500.28±25.73	4.86±0.14	47.06±0.64	497.99±0.64	4.77±0.11	46.60±0.72	506.54±3.36
%RSD	1.71	2.49	1.71	2.22	1.71	1.37	4.60	1.07	0.86	2.85	0.61	5.14	2.96	1.37	1.16	2.38	1.55	0.66
Accuracy	98.82	96.97	98.82	109.39	105.96	113.84	91.56	91.75	115.42	97.29	93.55	100.06	97.26	94.13	99.60	95.40	93.19	101.31
11-OH-THC
Mean±SD	5.04±0.03	53.38±0.22	529.27±5.06	5.88±0.01	59.10±0.82	597.42±1.12	4.70±0.05	51.01±0.26	531.94±4.77	5.19±0.10	52.72±0.03	545.50±19.65	5.08±0.07	53.04±0.26	549.62±6.69	5.17±0.06	52.56±0.26	553.07±4.97
%RSD	0.53	0.42	0.96	0.25	1.38	0.19	1.12	0.51	0.90	2.00	0.06	3.60	1.46	0.50	1.22	1.20	0.49	0.90
Accuracy	100.84	106.75	105.85	117.56	118.20	119.48	93.98	102.01	106.39	103.75	105.44	109.10	101.56	106.28	109.92	103.36	105.12	110.61
11-COOH-THC
Mean±SD	4.86±0.09	52.02±1.39	521.66±9.88	5.73±0.08	56.26±0.47	590.14±5.93	4.69±0.16	47.40±0.37	562.61±2.49	4.92±0.11	49.04±0.01	515.85±31.28	4.90±0.10	49.19±0.37	533.81±9.52	4.93±0.13	49.13±0.54	527.35±2.11
%RSD	1.88	2.67	1.89	1.48	0.83	1.00	3.44	0.79	0.44	2.17	0.03	6.06	2.11	0.74	1.78	2.55	1.09	0.40
Accuracy	97.29	104.04	104.33	114.68	112.52	118.03	93.79	94.80	112.52	98.45	98.08	103.17	98.10	98.37	106.76	98.51	98.27	105.47