Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 13;30(3):102–120. doi: 10.1089/hgtb.2019.039

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Olaf Oberschmidt, et al. 2019; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are cited.

PMC Copyright notice

Figure 3. — Comparison of cytotoxic properties and activation markers on primary NK cells before and after the expansion process in Prodigy. IPCs (approximately 2.0–2.5 mL) were collected as indicated in Fig. 1A for analysis of NK cell-dependent cytotoxicity, CD107a degranulation, surface marker expression levels, and cytokine secretion. NK cell phenotyping of (A) NCRs (NKG2D, NKp30, NKp44, and NKp46), (B) activation markers (CD69 and CD137), and death receptors (CD178 and CD253) were analyzed by flow cytometry for 2-week-cultured NK cells compared to highly purified NK cells before the expansion period. Correspondingly, effector cell-mediated cytotoxicity (C) and CD107a degranulation rates (D) were analyzed for expanded NK cells against K562 cells at indicated effector-to-target (E/T) ratios and compared to freshly separated NK cells before starting the expansion period (day 0). (E) Secretion of tumor necrosis factor alpha, interferon gamma, perforin, granzyme A and B, and granulysin were determined after cytolytic reactions in supernatants of co- and mono-cultured NK and K562 cells. Medians with ranges are shown from three independent experiments performed in duplicate.