. 2019 Jul;25(7):1330–1338. doi: 10.3201/eid2507.181614

Table 1. Primer sequences used for PCRs to screen and genotype samples collected in study of genetic diversity of Plasmodium falciparum parasites, North Central Nigeria, 2015–2018*.

PCR description	Primer name or type: sequence, 5′→3′	Size, bp	Reference
Nest-1	rPLU1: TCAAAGATTAAGCCATGCAAGTGA	620	(27)
Nest-1	rPLU5: CCTGTTGTTGCCTTAAACTCC	620	(27)
Nest-2, genus-specific PCR	rPLU3: TTTTTATAAGGATAACTACGGAAAAGCTGT	240	(27)
Nest-2, genus-specific PCR	rPLU4: TACCCGTCATAGCCATGTTAGGCCAATACC	240	(27)
Plasmodium falciparum species–specific PCR	rFAL1: TTAAACTGGTTTGGGAAAACCAAATATATT	205	(27)
Plasmodium falciparum species–specific PCR	rFAL2: ACACAATGAACTCAATCATGACTACCCGTC	205	(27)
msp1, primary reaction	Forward: CTAGAAGCTTTAGAAGATGCAGTATTG	Variable	(28)
msp1, primary reaction	Reverse: CTTAAATAGTATTCTAATTCAAGTGGATCA	Variable	(28)
K1	Forward: AAATGAAGAAGAAATTACTACAAAAGGTGC	Variable	(28)
K1	Reverse: GCTTGCATCAGCTGGAGGGCTTGCACCAGA	Variable	(28)
MAD20	Forward: AAATGAAGGAACAAGTGGAACAGCTGTTAC	Variable	(28)
MAD20	Reverse: ATCTGAAGGATTTGTACGTCTTGAATTACC	Variable	(28)
RO33	Forward: TAAAGGATGGAGCAAATACTCAAGTTGTTG	Variable	(28)
RO33	Reverse: CATCTGAAGGATTTGCAGCACCTGGAGATC	Variable	(28)
msp2, primary reaction	Forward: ATGAAGGTAATTAAAACATTGTCTATTATA	Variable	(28)
msp2, primary reaction	Reverse: CTTTGTTACCATCGGTACATTCTT	Variable	(28)
FC27	Forward: AATACTAAGAGTGTAGGTGCARATGCTCCA	Variable	(28)
FC27	Reverse: TTTTATTTGGTGCATTGCCAGAACTTGAAC	Variable	(28)
IC/3D7	Forward: AGAAGTATGGCAGAAAGTAAKCCTYCTACT	Variable	(28)
IC/3D7	Reverse: GATTGTAATTCGGGGGATTCAGTTTGTTCG	Variable	(28)
glurp, primary reaction	Forward: TGAATTTGAAGATGTTCACACTGAAC	Variable	(28)
glurp, primary reaction	Reverse: GTGGAATTGCTTTTTCTTCAACACTAA	Variable	(28)
glurp	Forward: TGTTCACACTGAACAATTAGATTTAGATCA	Variable	(28)
glurp	Reverse: GTGGAATTGCTTTTTCTTCAACACTAA	Variable	(28)

*glurp, glutamate-rich protein; msp1, merozoite surface protein 1; msp2, merozoite surface protein 2.