Skip to main content
. 2019 Jun 12;25(11-12):842–854. doi: 10.1089/ten.tea.2018.0067

FIG. 1.

FIG. 1.

Development and properties of PADS from human AT explants. (A) Representative image of an explant from human subcutaneous AT (top left corner of each panel) in culture for the time indicated above each panel. (B) Higher magnification of specific section of well, taken at the times indicated. At week 14, Mitotracker red was added and cells imaged after 30 min. (C) Mean adipocyte size over time, from explants grown in the presence or absence of additional insulin added to the culture medium (100 nM). Symbols are the mean and bars are SEM of adipocyte sizes, obtained from five independent wells per time point as described in Materials and Methods. Statistical significance was estimated using two-way ANOVA with Dunnett's correction for multiple comparisons. *p < 0.05, ****p < 0.0001 versus week 7; ####p < 0.0001 versus no insulin. (D) Concentration of leptin in media after 24 h of culture at week 14. Shown are means and SEM of 8–10 wells. Statistical significance was estimated using two-tailed unpaired Mann–Whitney test. ***p < 0.001. ANOVA, analysis of variance; AT, adipose tissue; PADS, primed ADipose progenitor cells; SEM, standard error of the mean.