FIG. 2.

Comparison of grafts formed from ad-PADS and from liposuction tissue. (A) Micro-CT scan of mice implanted with PADS (23 weeks post-implantation, top panel) or liposuction tissue (7 weeks post-implantation, bottom panel). Areas of low attenuation are enclosed by red dotted lines. (B) Levels of human adiponectin in plasma from mice implanted as in (A). Each symbol represents the value for each mouse in the cohort. (C) Ratio of implant area versus circulating adiponectin. Each symbol represents one mouse, and lines represent the mean and SEM. Statistical significance was estimated using a two-tailed unpaired t-test with Welch's correction for unequal standard deviations. *p < 0.05. (D) Histochemical staining of implants formed from PADS (top panels) or liposuction tissue (bottom panels). Expanded regions illustrate the algorithm output for selection of particles used for quantification. (E) Histogram of particle sizes obtained from the mean values of five independent grafts from ad-PADS and seven independent grafts from liposuction tissue. Visual inspection was used to estimate adipocytes as those particles with values lower than between 100 and 5000 μm². Statistical significance was estimated using the Friedman test of frequency distributions as implemented in Prism 7.0. ****p < 0.0001. CT, computed tomography.