FIG. 5.

Quantification of graft volume, functionality, and endothelial or adipose cell content. (A) Mean and SEM of graft volumes calculated from micro-CT scans, and (B) mean and SEM of human adiponectin in plasma, obtained at the times shown on the x-axes, from seven mice per condition implanted with liposuction tissue, ad-PADS, or liposuction tissue supplemented by ad-PADS. Statistical significance was estimated using paired t-tests. *p < 0.05, **p < 0.01. (C) Mean and SEM of the ratios of human adiponectin to graft volumes at 16 weeks postgrafting in mice harboring grafts from liposuction tissue, ad-PADS, or liposuction tissue supplemented by ad-PADS. Statistical significance was estimated using one-way ANOVA with Tukey's correction for multiple comparisons, **p < 0.01. (D) Relationship between graft volume and plasma adiponectin in mice from cohorts 1 (squares) and 2 (circles). Each symbol corresponds to values from one mouse. (E–H) RNA from excised grafts was analyzed using species-specific qRT-PCR probes for the genes indicated above each panel. Bars represent the mean and lines the SEM of all grafts. Statistical significance was estimated using the Krustal–Wallis test with Dunnett's correction for multiple comparisons. **p < 0.01, ***p < 0.001, ****p < 0.00001. (I) Whole-mount staining of fragments from control human and mouse AT, and from grafts formed from liposuction tissue, ad-PADS, or liposuction tissue supplemented by ad-PADS, costained with mouse- and human-specific lectins and DAPI. DAPI, 4′,6-diamidino-2-phenylindole (nuclear staining); mRNA, messenger RNA; qRT-PCR, quantitative real time polymerase chain reaction.