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. 2019 Jun 12;25(11-12):842–854. doi: 10.1089/ten.tea.2018.0067

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© Raziel Rojas-Rodriguez et al., 2019; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are cited.

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FIG. 6. — Molecular features associated with tissue formation. (A) Schematic representing the methods to obtain PADS, ad-PADS, ADSCs, and ad-ADSCs. (B, C) RNA from two independent preparations of PADS, ad-PADS, ADSCs, and ad-ADSCs was probed for the mesenchymal stem cell (B, C) and adipocyte (C) markers depicted on the x-axis. Values are expressed as the fold over the lowest value for each probe set. Statistical significance was estimated using multiple t-tests corrected for multiple comparisons using the Holm-Sidak method. Double headed arrows are placed over sets of probes that differed significantly, at the levels indicated **p < 0.01, ***p < 0.001, ****p < 0.00001. (D) Oil Red O staining of ad-ADSCs and ad-PADS after 10 days of differentiation. (E) Whole-mount costaining of grafts formed from ADSCs and PADS using DAPI, and mouse- and human-specific lectins. Control human and mouse AT are included for comparison. Linear structures stained with human-specific lectin in grafts formed from ADSCs are indicated with red arrows. (F) RNA from excised grafts was analyzed using species-specific qRT-PCR probes for the genes indicated on the x-axis. Bars represent the mean, and lines the SEM of seven grafts per condition assayed in duplicate. Statistical significance was estimated using the Krustal–Wallis test with Dunnett's correction for multiple comparisons. **p < 0.01. (G) Heat map of differentially expressed genes between PADS and ad-PADS, obtained from AT from five different individuals. (H) Heat map of differentially expressed genes containing the GO term “extracellular.” ADSCs, ADipose stem cells.