Table 1. Toxicity of subfractions of the butanol fraction from first reversed-phase flash chromatography against newborn (< 24 h old) Acyrthosiphon pisum nymphs following 24 h exposure to artificial diet containing different concentrations of the subfractions.
| Fractions | LC50 (95% CI) ppm | Ratio | LC90 (95% CI) ppm | Ratio | Slope ± SE | Chi-Square | HF |
|---|---|---|---|---|---|---|---|
| 1A | 5.5 (3–8) a | 2.6 | 66 (37–211) a | 2.2 | 1.1 ± 0.3 | 7.1 | 0.5 |
| 2A | 8.9 (6.1–12) a | 4.2 | 81 (47–231) a | 2.7 | 1.3 ± 0.3 | 5.6 | 0.4 |
| 3A | 2.1 (0.6–3.8) a | 1.0 | 30 (18–85) a | 1.0 | 1.1± 0.3 | 7.5 | 0.6 |
| 4A | 6.8 (3.8–10) a | 3.2 | 112.2 (54–561) a | 3.8 | 1.1 ± 0.3 | 4.6 | 0.4 |
| 5A | 3.3 (1.3–5.4) a | 1.6 | 50 (28–176) a | 1.7 | 1.1 ± 0.3 | 10.1 | 0.8 |
| 6A | 18 (13–27) b | 8.5 | 187 (90–808) a | 6.3 | 1.3 ± 0.3 | 3.8 | 0.3 |
| 7A | 74 (52–169) c | 35.3 | 267 (131–1651) a | 9.1 | 2.3 ± 0.6 | 8.1 | 0.6 |
| 8A | - | - | - | - | 1.7 ± 0.7 | 7.0 | 0.5 |
| 9A | - | - | - | - | 2.0 ± 1.3 | 4.7 | 0.4 |
| 10A | 36 (33–40) d | 17.2 | 52.5 (46–64) a | 1.8 | 8.0 ± 1.4 | 2.8 | 0.2 |
| 11A | - | - | - | - | 1.6 ± 0.6 | 8.5 | 0.7 |
| 12A | 51 (43–71) c | 24.5 | 109 (77–241) a | 3.7 | 3.9 ± 1.0 | 2.2 | 0.2 |
| 13A | - | - | - | - | 1.5 ± 1.2 | 6.6 | 0.5 |
| 14A | - | - | - | - | 2 ± 1.3 | 4.7 | 0.4 |
Data is presented as lethal concentration values, 50% (LC50) and 90% (LC90) (both in ppm) together with their particular 95% confidence interval (95% CI), the slope ± SE of the toxicity vs concentration curve, and the Chi-Square and heterogeneity factor HF as accuracy of data fitting to probit analysis in POLO-PlusV2. Due to non-overlapping of 95% CI, different letters in the same column indicate significant differences. Ratio, LCx, fraction/LCx, 3A