Table 3. Toxicity of subfractions of the butanol fraction from a second reversed-phase flash chromatography against newborn (<24 h old) Acyrthosiphon pisum nymphs following 24 h exposure to artificial diet containing different concentrations of the subfractions.
| Fractions | LC50 (95% CI) ppm | Ratio | LC90 (95% CI) ppm | Ratio | Slope ± SE | Chi-Square | HF |
|---|---|---|---|---|---|---|---|
| 1B | 2.5 (1–4.1) a | 1.0 | 28 (18–69) a | 1 | 1.2 ± 0.3 | 11.4 | 0.9 |
| 2B | 7.5 (4.3–11) b | 3.0 | 71 (38–280) a | 2.5 | 1.3 ± 0.3 | 16.5 | 1.3 |
| 3B | 16 (11–26) c | 6.5 | 101 (52–417) a | 3.6 | 1.6± 0.3 | 22.3 | 1.7 |
| 4B | - | - | - | - | 1.0 ± 0.3 | 25.3 | 2.0 |
| 5B | - | - | - | - | 1.5 ± 1.2 | 6.6 | 0.5 |
| 6B | - | - | - | - | 1.8 ± 0.7 | 6.5 | 0.5 |
Data is presented as lethal concentration values, 50% (LC50) and 90% (LC90) (both in ppm) together with their particular 95% confidence interval (95% CI), the slope ± SE of the toxicity vs concentration curve, and the Chi-Square and heterogeneity factor HF as accuracy of data fitting to probit analysis in POLO-PlusV2. Due to non-overlapping of 95% CI, different letters in the same column indicate significant differences. Ratio, LCx, fraction/LCx, 1B