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Binding of selected DARPin monomers, pooled monomers, or dimers to TcdB was determined using ELISA. Combinations of serially diluted DARPins were added to microtiter plates coated with 4 μg/mL of TcdB. Results are representative of 2 independent experiments. Conc, concentration; DARPin, designed ankyrin repeat protein; ELISA, enzyme-linked immunosorbent assay; TcdB, C. difficile toxin B.
