Fig 3. Zonal gradient centrifugation.
Zonal centrifugation of Strep-HP1542 (upper panel) and gel filtration standard mix (Bio Rad, lower panel) through 5–15% sucrose gradient. Equal amounts of sample fraction were loaded on SDS-PAGE and stained with Coomassie. Strep-HP1542 containing fractions and standard proteins were indicated by arrowheads and names respectively.