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. 2019 Jun 24;14(6):e0218474. doi: 10.1371/journal.pone.0218474

Fig 7. Secondary structure analysis.

Fig 7

(A) In silico prediction of the secondary structure of HP1542, as created by the PSIPRED server. The Bactofillin domain (DUF583) is illustrated as orange passage, β-sheets as green arrows and α-helices as orange tube. (B) CD spectroscopy of Strep-HP1542. CD-units are plotted in mDeg against the wavelength in 190–250 nm for HP1542 under mild (blue) and acidic (red) conditions as well as for Poly-L-Lysin in pH 12 (green). The calculated secondary structure contributions are illustrated in pie charts. (C) SDS-PAGE analysis of the digestion of Strep-HP1542 with proteinase K (Sigma-Aldrich) at the indicated concentrations (mg ml−1).