| Strains: H. pylori |
| Name |
Description |
reference |
| 26695 |
wt |
[27] |
| G27 |
wt |
[28] |
| 1061 |
wt |
[29] |
| 26695_1542KM |
26695 hp1542::Pneo
|
This study |
| G27_1480KM |
G27 hp1542::Pneo
|
This study |
| 1061_1542KM |
1061 hp1542::Pneo
|
This study |
| Strains: E. coli |
| Name |
Description |
reference |
| DH5α |
E. coli K12 derivate, fhuA2 lac(del)U169 phoA glnV44 Φ80' lacZ(del)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17. Used for molecular cloning |
Bethesda Research Laboratories |
| BL21(DE3) |
E. coli BL21 derivate, dcm ompT hsdS(rB-mB-) gal carrying the λDE3 prophage. Used as host for heterologous expression of H. pylori genes |
Stratagene |
| Primer |
| Name |
Sequence |
reference |
| HPG27_1480-80u-5 |
CAG CCC ATA AAC CCC ATG AGT |
[22] |
| NEO-1542-L1 |
CCT AGA TTT AGA TGT CTG GAA ACT CGC CCT AAG AAT A |
This study |
| 1542-PNEO-R1 |
CGT ACC GGT TCC AAT TTT GCC ATC TAC ATG CAA ATG GT |
This study |
| HP1542up BsaI |
ATG GTA GGT CTC AGC GCA TGG CAA TCT TTG ATA ACA ATA ATA AAT |
This study |
| HP1542dw BsaI |
ATG GTA GGT CTC ATA TCA TTT ATT TTC AAT TTT CTT TTC TTG CTC A |
This study |
| Hp1542_F: |
AAT TCC ATG GGC GCA ATC TTT GAT AAC |
This study |
| Hp1542-6H_R |
AAT TGG ATC CTT AAT GGT GAT GGT GAT GGT GTT TAT TTT CAAT TTT |
This study |
| 1542_112_AUG_BsaI_for |
ATG GTA GGT CTC AGC GCA TGC ACG TAG ATG GCG AAT TAG |
This study |
| 1542_339_BsaI_rev |
ATG GTA GGT CTC ATA TCT ATT CCC CAA TCA AAA TCC CC |
This study |
