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. 2019 Jun 24;14(6):e0218474. doi: 10.1371/journal.pone.0218474

Table 2. List of plasmids used in this study.

Plasmids
Name Relevant characteristics Reference
pASK7_1542 pASK-iba7 expression vector carrying hp1542 ORF under control of the Tet-promotor, inserted in BsaI restriction site This study
pET24d-Hp1542-6HC Pet-24d expression vector carrying hp1542 ORF, fused to the sequence of a 6-His tag, under control of the T7-promotor, inserted between NcoI and XhoI restriction sites This study
pZErO-2 Cloning vector, MCS in lacZ_ neo, Kmr Invitrogen
pZErO-2_hp1480 pZEro-2 containing the Sequence of hp1480 for hp1480 (hp1542) deletions by vector integration through single cross-over This study
pASK7_1542_1–339 pASK-iba7 expression vector carrying bp 1 to 339 of hp1542 ORF under control of the Tet-promotor, inserted in BsaI restriction site This study
pASK7_1542_112–411 pASK-iba7 expression vector carrying bp 112 to 411 of hp1542 ORF under control of the Tet-promotor, inserted in BsaI restriction site This study