Table 3. Buffer-compositions for HP1542 low spin down assay.
Description | Buffer composition |
---|---|
NaCl | 50 mM Tris pH 7,5; 0,5–1,5 M NaCl |
KCl | 50 mM Tris pH 7,5; 0,5–1,5 M KCl |
Urea | 50 mM Tris pH 7,5; 50 mM NaCl; 0,5 M Urea |
Arg/Glu | 50 mM Tris pH 7,5; 50 mM NaCl; 50 mM L-Arg; 50 mM L-Glu |
pH 5 | 50 mM C2H3NaO2, 50 mM NaCl, 5 mM EDTA |
pH 6 | 50 mM MES, 50 mM NaCl, 5mM EDTA |
pH 7,5 | 50 mM Tris, 50 mM NaCl, 5 mM EDTA |
pH 8 | 50 mM Tris, 50 mM NaCl, 5 mM EDTA |
pH 9 | 50 mM Tris, 50 mM NaCl, 5 mM EDTA |