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. 2019 Jun 24;14(6):e0218474. doi: 10.1371/journal.pone.0218474

Table 3. Buffer-compositions for HP1542 low spin down assay.

Description Buffer composition
NaCl 50 mM Tris pH 7,5; 0,5–1,5 M NaCl
KCl 50 mM Tris pH 7,5; 0,5–1,5 M KCl
Urea 50 mM Tris pH 7,5; 50 mM NaCl; 0,5 M Urea
Arg/Glu 50 mM Tris pH 7,5; 50 mM NaCl; 50 mM L-Arg; 50 mM L-Glu
pH 5 50 mM C2H3NaO2, 50 mM NaCl, 5 mM EDTA
pH 6 50 mM MES, 50 mM NaCl, 5mM EDTA
pH 7,5 50 mM Tris, 50 mM NaCl, 5 mM EDTA
pH 8 50 mM Tris, 50 mM NaCl, 5 mM EDTA
pH 9 50 mM Tris, 50 mM NaCl, 5 mM EDTA