Fig 6. Overexpression of podoplanin imbues NHDFs not activated with MDA-MB-231 conditioned medium with the capacity to accelerate aggregation and coalescence, and pretreatment of CC-NHDFs with anti-podoplanin mAb blocks its capacity to accelerate.

A. Aggregation and aggregate coalescence of MDA-MB-231 preparations after 24 hours in which the substratum was NHDF cells on collagen. B. Aggregation and aggregate coalescence of MDA-MB-231 preparations after 24 hours in which the substratum was NHDF-PDPN^œ cells on collagen. C, D. Aggregation and coalescence of MDA-MB-231 at 72 hours, in which the substratum was either NHDF (C) or NHDF-PDPN^œ cells (D) on collagen. E, F. Aggregation and aggregate coalescence of MDA-MB-231 after 72 hours in which the substratum was either untreated CC-NHDFs (E) or CC-NHDFs pretreated with the anti-podoplanin mAb NZ-1 (F).